Cstps1
WebDec 10, 2014 · The coding sequence of (+)-valencene synthase CsTPS1 ( C. sinensis) was obtained from NCBI GeneEntrez Database (GenBank: AF441124.1 ). The sequence was codon optimized for C. glutamicum by the Java Codon Adaptation Tool (JCat) ( Grote et al., 2005) and the optimized gene was synthesized by GeneArt ® life technologies™ … WebBiochemical goals • In Citrus sinensis, the Cstps-1 gene codes for the production of the enzyme Citrus Valancene Synthase (CVS). • In vivo, CVS reacts with farnesyl pyrophosphate (FPP) to produce the characteristically fragrant valencene (as well as 5 …
Cstps1
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WebDec 15, 2024 · However, CsTPS1 and CsTPS6 were down-regulated with the NaCl treatment and reached the lowest expression level at 24 h. Under H 2 O 2 treatment, … WebNon-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains …
WebSep 1, 2011 · Expression of a cytosolic form of CsTPS1 in addition to mtCsTPS1 further elevated valencene production levels by approximately 25%, an amount (300 μg/l) that … WebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. …
WebMar 6, 2015 · Two thirds of differentially expressed proteins were identified in the pathways of glycolysis, citric acid cycle, amino acid, sugar and starch metabolism. An enzyme encoding valencene synthase gene ( Cstps1) was … Webthat CitAP2.10 could trans-activate the CsTPS1 promoter. Ethylene enhanced expression of CitAP2.10 and this effect was abolished by the ethylene antagonist 1 …
WebOct 19, 2024 · CsTPS1-3 have predicted chloroplast-target peptides and produced predominantly linalool when GPP was supplied as substrate. Furthermore, CsTPS9 catalysed the formation of ( E )-β-ocimene and myrcene, and small amounts of sabinene and ( Z )-β-ocimene from GPP. fish hook remover reviewsCsTPS1 has previously been characterized as the key gene encoding the enzyme terpene synthase that catalyzes (+)-valencene biosynthesis (Sharon-Asa et al., 2003). Therefore, we screened a large set of citrus transcription factors for activation or inhibition using the promoter of CsTPS1 and a dual-luciferase assay ( Supplementary Fig. S1 ). fish hook removal toolsWebMar 13, 2024 · Results. There was a clear relationship between presence or absence of the valencene synthase gene (Cstps1) expression, and presence or absence of valencene among randomly selected mandarin hybrids.Cloning the coding regions of Cstps1 from Fortune and Murcott mandarin, and aligning with previous reported Valencia orange … can a tfsa be held in trustWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. fish hook remover stainless steelWebJul 28, 2012 · The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR … can atf be used in power steeringWebCsTPS1- AS reduced the accumulation of geraniol, and the silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. However, the geraniol content and pathogen resistance of CsTPS1-silenced plants and control plants did not signi cantly di er. Further analysis showed that silencing of CsTPS1- AS led to a ... canathaWebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene synthases. fish hook removal with string