Immortalized human podocyte cell line
WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture. This simplifies analysis of the biology of cells which may otherwise have a limited lifetime.
Immortalized human podocyte cell line
Did you know?
Witryna14 lut 2015 · Cell culture and high glucose-induced podocytopathy: An immortalized mouse podocyte cell line 25 which had been generated by expression of a temperature-sensitive mutant of the SV40 Large-T ... WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Witryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of … WitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these …
WitrynaImmortalized Primary Cells. Innoprot offer Human and animal primary cells immortalized with SV40 antigen T and other immortalization techniques. All immortalized cell lines has been characterized for specific markers and other for functional assays. Showing 1–16 of 23 results. 1 2. Witryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. …
Witryna17 wrz 2024 · Generally, conditionally immortalized podocytes have lost many of their phenotypes in vivo, including process formation at high cell density with conventional …
WitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation. chinese auto glass company dayton ohioWitrynaSteroid-resistant nephrotic syndrome (SRNS) is a frequent cause of CKD. The discovery of monogenic causes of SRNS has revealed specific pathogenetic pathways, but these monogenic causes do not explain all cases of SRNS. To identify novel monogenic grand champion nuzzling horseWitrynaOther investigators have immortalized mouse and human neural stem cells using retroviral transduction of the v-myc oncogene (Snyder, 1995; ... 2007) and his team … chinese automation congress cac 2020WitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, … chinese autism awareness centerWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein levels and increased S1P levels. chinese automakers in usWitrynaLoss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled bran… chinese automation congress cac 影响因子Witryna1 kwi 2002 · The conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [8, 40]. Podocytes were cultured in the medium which contained ... chinese auto manufacturers